- Code CHEM2208
- Unit Value 6 units
- Offered by Research School of Chemistry
- ANU College ANU Joint Colleges of Science
- Course subject Chemistry
- Areas of interest Chemistry
- Academic career UGRD
- Prof Thomas Huber
- Mode of delivery In Person
Second Semester 2022
See Future Offerings
In Sem 2 2022, this course is on campus with remote adjustments only for participants with unavoidable travel restrictions/visa delays.
Many applications in modern chemistry and biochemistry depend on the ability to make, change and analyze proteins. After completion of the course, students will be familiar with all of the steps required for the production of proteins in bacteria, protein structural features and techniques for making, modifying (including the site-specific introduction of non-natural amino acids), and analyzing proteins. There is an emphasis on biophysical techniques to characterize biomolecules (e.g., SDS-PAGE, light scattering, circular dichroism spectroscopy, ultracentrifugation, mass spectroscopy, surface plasmon resonance), including discussion of their physical basis. Advanced structural analysis techniques (X-ray crystallograhy, NMR spectroscopy, electron microscopy, solution scattering) are also discussed. An introduction to bioinformatics, i.e. protein sequence alignment, 3D structure analysis and modelling is also included.
Upon successful completion, students will have the knowledge and skills to:
- Understand the necessary elements of protein over-expression systems in bacteria,
- Make and purify proteins,
- Understand elements of peptide and protein structure and function,
- Understand techniques for modifying proteins,
- Demonstrate familiarity with basic techniques for protein analysis,
- Understand advanced biophysical techniques for protein analysis, including the capacity to discuss their relative merits and interpret data from those techniques,
- Demonstrate familiarity with software for protein visualization, sequence alignment and modelling.
- Laboratory and workshop based practicals (7 in total, 5% each). (35) [LO 2,4,5,7]
- Mid-semester exam and final exam (each worth 32.5%) (65) [LO 1,3,4,6]
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The expected workload will consist of approximately 130 hours throughout the semester including:
- Face-to face component which may consist of 3 x 1 hour lectures and 1 x 1 tutorial per week; and 7 x 3-4 hour practicals across the semester.
- Approximately 73 hours of self directed study which will include preparation for lectures, practicals and other assessment tasks.
To be determined
Requisite and Incompatibility
Selected chapters from 'Biochemistry' by Voet & Voet, 4th edition
Selected readings will be provided by individual lecturers
Tuition fees are for the academic year indicated at the top of the page.
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- 6 units
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