• Offered by Research School of Chemistry
  • ANU College ANU Joint Colleges of Science
  • Course subject Chemistry
  • Areas of interest Chemistry
  • Academic career UGRD
  • Course convener
    • Prof Gottfried Otting
  • Mode of delivery In Person
  • Co-taught Course
  • Offered in Second Semester 2014
    See Future Offerings

Many applications in modern chemistry and biochemistry depend on the ability to make, change and analyse proteins and enzymes. After completion of the course students will be familiar with all of the steps required for the production of proteins in bacteria and various techniques of analysis. This includes techniques for making, modifying, and analysing proteins.  There is an emphasis on biophysical techniques (SDS-PAGE, light-scattering, CD spectroscopy, ultracentrifugation, mass spectrometry, ITC, fluorescence, surface plasmon resonance), discussing their physical basis in depth. Advanced analysis techniques (X-ray crystallography, NMR, EM, SAXS/SANS) are discussed only superficially.  Introduction to bioinformatics: protein sequence alignment, 3D structure analysis, modelling.

Learning Outcomes

Upon successful completion, students will have the knowledge and skills to:

After successful completion of the course students will have:

  1. Understanding of the necessary elements of protein overexpression systems in bacteria,
  2. Capability to design all the steps required to produce an expression system for a new protein,
  3. Capability to make and purify proteins,
  4. Understanding of techniques for modifying proteins,
  5. Experience with basic techniques for protein analysis,
  6. Understanding of advanced biophysical techniques for protein analysis, including the capacity to discuss their relative merits and interpret data from those techniques,
  7. Familiarity with software for protein visualization, sequence alignment and modelling. 

Indicative Assessment

50% by written exam, 50% by weekly assessments of practicals and tutorials (10 in total, 5% each). Students have to pass the exam to pass the course.

The ANU uses Turnitin to enhance student citation and referencing techniques, and to assess assignment submissions as a component of the University's approach to managing Academic Integrity. While the use of Turnitin is not mandatory, the ANU highly recommends Turnitin is used by both teaching staff and students. For additional information regarding Turnitin please visit the ANU Online website.

Workload

The course comprises 26 lectures (1 hour each), 6 practicals (3 hours each) and 8 tutorial/quiz sessions (1 hour each).

Practicals are 3-4 hours each. Each practical requires about 1 hour of preparation and 1 hour of reporting. Assessments require independent reading for about 1 hour per contact hour. 

Requisite and Incompatibility

To enrol in this course you must have completed CHEM1101, CHEM1201 and BIOL1004

Prescribed Texts

Selected chapters from Biochemistry by Voet & Voet

Majors

Specialisations

Fees

Tuition fees are for the academic year indicated at the top of the page.  

If you are a domestic graduate coursework or international student you will be required to pay tuition fees. Students continuing in their current program of study will have their tuition fees indexed annually from the year in which you commenced your program. Further information for domestic and international students about tuition and other fees can be found at Fees.

Student Contribution Band:
2
Unit value:
6 units

If you are an undergraduate student and have been offered a Commonwealth supported place, your fees are set by the Australian Government for each course. At ANU 1 EFTSL is 48 units (normally 8 x 6-unit courses). You can find your student contribution amount for each course at Fees.  Where there is a unit range displayed for this course, not all unit options below may be available.

Units EFTSL
6.00 0.12500
Domestic fee paying students
Year Fee
1994-2003 $1650
2004 $1926
2005 $2298
2006 $2520
2007 $2520
2008 $2916
2009 $2916
2010 $2916
2011 $2946
2012 $2946
2013 $2946
2014 $2946
International fee paying students
Year Fee
1994-2003 $3390
2004 $3450
2005 $3450
2006 $3618
2007 $3618
2008 $3618
2009 $3618
2010 $3750
2011 $3756
2012 $3756
2013 $3756
2014 $3762
Note: Please note that fee information is for current year only.

Offerings, Dates and Class Summary Links

The list of offerings for future years is indicative only.
Class summaries, if available, can be accessed by clicking on the View link for the relevant class number.

Second Semester

Class number Class start date Last day to enrol Census date Class end date Mode Of Delivery Class Summary
8149 21 Jul 2014 01 Aug 2014 31 Aug 2014 30 Oct 2014 In Person N/A

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