This course includes an on campus activity/ies. Check timetable for details. Contact course convener if you are unable to travel to Canberra.
Many applications in modern chemistry and biochemistry depend on the ability to make, change and analyse proteins and enzymes. After completion of the course students will be familiar with all of the steps required for the production of proteins in bacteria and various techniques of analysis. This includes techniques for making, modifying, and analysing proteins. There is an emphasis on biophysical techniques (SDS-PAGE, light-scattering, CD spectroscopy, ultracentrifugation, mass spectrometry, ITC, fluorescence, surface plasmon resonance), discussing their physical basis in depth. Advanced analysis techniques (X-ray crystallography, NMR, EM, SAXS/SANS) are discussed only superficially. Introduction to bioinformatics: protein sequence alignment, 3D structure analysis, modelling.
Upon successful completion, students will have the knowledge and skills to:
After successful completion of the course students will have:
- Understanding of the necessary elements of protein overexpression systems in bacteria,
- Capability to design all the steps required to produce an expression system for a new protein,
- Capability to make and purify proteins,
- Understanding of techniques for modifying proteins,
- Experience with basic techniques for protein analysis,
- Understanding of advanced biophysical techniques for protein analysis, including the capacity to discuss their relative merits and interpret data from those techniques,
- Familiarity with software for protein visualization, sequence alignment and modelling.
50% by written exam, 50% by weekly assessments of practicals and tutorials (10 in total, 5% each). Students have to pass the exam to pass the course.
In response to COVID-19: Please note that Semester 2 Class Summary information (available under the classes tab) is as up to date as possible. Changes to Class Summaries not captured by this publication will be available via Wattle and/or students should have been advised by the offering College.
The course comprises 26 lectures (1 hour each), 6 practicals (3 hours each) and 8 tutorial/quiz sessions (1 hour each).
Practicals are 3-4 hours each. Each practical requires about 1 hour of preparation and 1 hour of reporting. Assessments require independent reading for about 1 hour per contact hour.
Requisite and Incompatibility
Selected chapters from Biochemistry by Voet & Voet
Tuition fees are for the academic year indicated at the top of the page.
- Student Contribution Band:
- Unit value:
- 6 units
Offerings, Dates and Class Summary Links
Class summaries, if available, can be accessed by clicking on the View link for the relevant class number.