- Code CHEM2208
- Unit Value 6 units
- Offered by Research School of Chemistry
- ANU College ANU Joint Colleges of Science
- Course subject Chemistry
- Areas of interest Chemistry
- Academic career UGRD
- Prof Gottfried Otting
- Mode of delivery In Person
Second Semester 2016
See Future Offerings
Many applications in modern chemistry and biochemistry depend on the ability to make, change and analyse proteins and enzymes. After completion of the course students will be familiar with all of the steps required for the production of proteins in bacteria and various techniques of analysis. This includes techniques for making, modifying, and analysing proteins. There is an emphasis on biophysical techniques (SDS-PAGE, light-scattering, CD spectroscopy, ultracentrifugation, mass spectrometry, ITC, fluorescence, surface plasmon resonance), discussing their physical basis in depth. Advanced analysis techniques (X-ray crystallography, NMR, EM, SAXS/SANS) are discussed only superficially. Introduction to bioinformatics: protein sequence alignment, 3D structure analysis, modelling.
Upon successful completion, students will have the knowledge and skills to:
After successful completion of the course students will have:
- Understanding of the necessary elements of protein overexpression systems in bacteria,
- Capability to design all the steps required to produce an expression system for a new protein,
- Capability to make and purify proteins,
- Understanding of techniques for modifying proteins,
- Experience with basic techniques for protein analysis,
- Understanding of advanced biophysical techniques for protein analysis, including the capacity to discuss their relative merits and interpret data from those techniques,
- Familiarity with software for protein visualization, sequence alignment and modelling.
50% by written exam, 50% by weekly assessments of practicals and tutorials (10 in total, 5% each). Students have to pass the exam to pass the course.
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The course comprises 26 lectures (1 hour each), 6 practicals (3 hours each) and 8 tutorial/quiz sessions (1 hour each).
Practicals are 3-4 hours each. Each practical requires about 1 hour of preparation and 1 hour of reporting. Assessments require independent reading for about 1 hour per contact hour.
Requisite and Incompatibility
Selected chapters from Biochemistry by Voet & Voet
Tuition fees are for the academic year indicated at the top of the page.
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- Student Contribution Band:
- Unit value:
- 6 units
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Offerings, Dates and Class Summary Links
ANU utilises MyTimetable to enable students to view the timetable for their enrolled courses, browse, then self-allocate to small teaching activities / tutorials so they can better plan their time. Find out more on the Timetable webpage.
Class summaries, if available, can be accessed by clicking on the View link for the relevant class number.
|Class number||Class start date||Last day to enrol||Census date||Class end date||Mode Of Delivery||Class Summary|
|8318||18 Jul 2016||29 Jul 2016||31 Aug 2016||28 Oct 2016||In Person||N/A|